Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections

Helicobacter. 2017 Dec;22(6). doi: 10.1111/hel.12430. Epub 2017 Aug 22.


Background: We have previously revealed that omega-3 polyunsaturated fatty acids can prevent Helicobacter pylori infection by blocking the futalosine pathway, an alternative route for menaquinone (MK) biosynthesis.

Materials and methods: 1, Different H. pylori strains were grown in liquid media supplemented with linoleic acid, an omega-6 fatty acid, or its 10-hydroxy derivative, 10-hydroxy-cis-12-octadecenoic acid (HYA), in the presence or absence of MK. The bacterial numbers in the media were estimated by plating; 2, C57BL/6NCrl mice received drinking water supplemented with different fatty acids starting from 1 week before infection with H. pylori or Helicobacter suis until the end of the experiment. The gastric colonization levels of H. pylori or H. suis were determined 2 weeks after infection by plating or quantitative PCR, respectively; 3, Mice were given HYA, starting 1 week before infection with H. suis and continuing until 6 months after infection, for analysis of the gastric conditions.

Results: 1, A low concentration (20 μmol/L) of HYA in culture broth suppressed the growth of H. pylori, and this inhibition was reduced by MK supplementation; 2, HYA treatment protected mice against H. pylori or H. suis infection; 3, HYA treatment suppressed the formation of lymphoid follicles in the gastric mucus layer after H. suis infection.

Conclusions: HYA prevents gastric Helicobacter infections by blocking their futalosine pathways. Daily HYA supplementation is effective for the prevention of gastric mucosa-associated lymphoid tissue lymphoma induced by persistent infection with H. suis.

Keywords: Helicobacter suis; alternative menaquinone biosynthesis; hydroxy fatty acid; mucosa-associated lymphoid tissue lymphoma.

MeSH terms

  • Animals
  • Bacterial Load
  • Colony Count, Microbial
  • Disease Models, Animal
  • Fatty Acids, Omega-6 / administration & dosage*
  • Female
  • Helicobacter Infections / drug therapy*
  • Helicobacter Infections / prevention & control*
  • Helicobacter heilmannii / drug effects
  • Helicobacter pylori / drug effects*
  • Mice, Inbred C57BL
  • Real-Time Polymerase Chain Reaction
  • Stearic Acids / administration & dosage*
  • Treatment Outcome
  • Vitamin K 2 / administration & dosage


  • Fatty Acids, Omega-6
  • Stearic Acids
  • Vitamin K 2
  • 10-hydroxystearic acid