A method for purification of bacterial R-type lipopolysaccharides (lipooligosaccharides)

Anal Biochem. 1987 Feb 1;160(2):281-9. doi: 10.1016/0003-2697(87)90048-0.


A new gel filtration method was developed for purification of R-type lipopolysaccharides (lipooligosaccharides) from some nonenteric gram-negative bacteria, including Neisseria meningitidis, Haemophilus influenzae, and Bordetella pertussis. These wild-type lipooligosaccharides are poorly extractable by the phenol-chloroform-ether extraction method of C. Galanos, O. Luderitz, and O. Westphal [1969) Eur. J. Biochem. 9, 245-249) and therefore a new procedure was developed for their isolation. The lipooligosaccharides (LOS) were first extracted by hot phenol-water, treated with RNase, then disaggregated in deoxycholic acid, and purified by gel filtration on Sephadex G-75. By comparison the conventional hot phenol-water purification method using repeated ultracentrifugations yielded less LOS. The yield of LOS by gel filtration was 30 to 108% higher and the purity was better.

MeSH terms

  • Bordetella pertussis / analysis
  • Chromatography, Gel / methods*
  • Gram-Negative Bacteria / analysis*
  • Haemophilus influenzae / analysis
  • Lipopolysaccharides / isolation & purification*
  • Neisseria meningitidis / analysis
  • Phenol
  • Phenols
  • Water


  • Lipopolysaccharides
  • Phenols
  • lipid-linked oligosaccharides
  • Water
  • Phenol