Portable kit for high-throughput analysis of polycyclic aromatic hydrocarbons using surface enhanced Raman scattering after dispersive liquid-liquid microextraction

Min Zhang; Xiaoli Zhang; Baofeng Qu; Jinhua Zhan

doi:10.1016/j.talanta.2017.07.072

Portable kit for high-throughput analysis of polycyclic aromatic hydrocarbons using surface enhanced Raman scattering after dispersive liquid-liquid microextraction

Talanta. 2017 Dec 1:175:495-500. doi: 10.1016/j.talanta.2017.07.072. Epub 2017 Jul 25.

Authors

Min Zhang¹, Xiaoli Zhang², Baofeng Qu², Jinhua Zhan³

Affiliations

¹ Key Laboratory for Colloid&Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China; School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
² Key Laboratory for Colloid&Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
³ Key Laboratory for Colloid&Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China. Electronic address: jhzhan@sdu.edu.cn.

PMID: 28842023
DOI: 10.1016/j.talanta.2017.07.072

Abstract

In this work, a portable kit was developed for convenient high-throughput trace analysis of polycyclic aromatic hydrocarbons (PAHs) using surface enhanced Raman scattering (SERS) after dispersive liquid-liquid microextraction (DLLME) process. This portable kit contains three sealed reagent tubes (labeled as T1, T2 and T3), a self-made well plate, and a portable Raman spectrometer. The reagent tube T1 contains a mixture of disperser solvent and extraction solvent, which involved a 2min sample pretreatment of DLLME process. The quick injection of solvents in tube T1 into the sample containing PAHs formed a cloudy solution immediately, which consists of fine droplets of extraction solvent dispersed entirely into aqueous phase. The enrichment factor was found to be 29.6. T2 and T3 contain methanol and 1-propanethiol-modified silver nanoparticles (PTH-Ag NPs), respectively. The liquid in the tube T3 was used to enhance the Raman signal of analytes on the self-made high-throughput micro reactor. A linear relationship between the concentration of pyrene and the relative Raman peak intensity was obtained (R² = 0.993). The detection limit was 0.50μgL^-1 for pyrene. RSD of the high-throughput analysis of 12 samples was calculated as 4.8%. The ability of DLLME-SERS technique in the extraction of PAH isomers from water samples were investigated. The performance of DLLME-SERS in the recovery of pyrene from lake, spring and drinking water was also studied.

Keywords: Dispersive liquid-liquid microextraction; High-throughput; Nanoparticles; Polycyclic aromatic hydrocarbons; Silver; Surface enhanced Raman spectroscopy.