Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay

J Neurosci Methods. 1987 May;20(1):83-90. doi: 10.1016/0165-0270(87)90041-0.


Measurement of lactate dehydrogenase (LDH) activity released to the extracellular bathing media has been found to be a simple yet quantitative method for assessing glutamate mediated central neuronal cell injury in cortical cell culture. Extracellular LDH is both chemically and biologically stable; the magnitude of LDH efflux in the cultures correlates in a linear fashion with the number of neurons damaged by glutamate exposure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cells, Cultured
  • Cerebral Cortex / drug effects*
  • Cerebral Cortex / enzymology
  • Fetus
  • Glutamates / pharmacology*
  • Glutamic Acid
  • L-Lactate Dehydrogenase / analysis*
  • Mice


  • Glutamates
  • Glutamic Acid
  • L-Lactate Dehydrogenase