Characterization of FaDu-R, a radioresistant head and neck cancer cell line, and cancer stem cells

Kwang-Jae Cho; Eun-Ji Park; Min-Sik Kim; Young-Hoon Joo

doi:10.1016/j.anl.2017.07.011

Characterization of FaDu-R, a radioresistant head and neck cancer cell line, and cancer stem cells

Auris Nasus Larynx. 2018 Jun;45(3):566-573. doi: 10.1016/j.anl.2017.07.011. Epub 2017 Aug 24.

Authors

Kwang-Jae Cho¹, Eun-Ji Park¹, Min-Sik Kim¹, Young-Hoon Joo²

Affiliations

¹ Department of Otolaryngology-Head & Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
² Department of Otolaryngology-Head & Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: joodoct@catholic.ac.kr.

PMID: 28844650
DOI: 10.1016/j.anl.2017.07.011

Abstract

Objectives: The aim of this study was to evaluate the impact of CSC on insensitivity to radiotherapy in HNSCC.

Methods: A radioresistant cell line, FaDu-R, was established using fractionated ionizing radiation. Cells with high and low CD44/ALDH activity were isolated.

Results: FaDu-R cells demonstrated significantly increased cell viability after radiation exposure compared with parental cells. CD44^high/ALDH^high FaDu-R cells demonstrated significantly faster wound closure (p<0.05) and more efficient invasion (p<0.05) compared to the CD44^high/ALDH^high FaDu cells or the CD44^low/ALDH^low FaDu-R cells. There was a significant difference in tumor volume between the CD44^high/ALDH^high FaDu-R cells and the CD44^high/ALDH^high FaDu cells (p<0.05) as well as the CD44^low/ALDH^low FaDu-R cells (p<0.05).

Conclusion: Cancer stem cells (CSC) were associated with invasion and tumorigenesis in a radioresistant head and neck squamous cell carcinoma (HNSCC) cell line. This concept might help to improve the understanding of these mechanisms and to develop drugs that can overcome radioresistance during radiotherapy.

Keywords: Head and neck neoplasms; Neoplastic stem cells; Radiation tolerance; Radiotherapy; Squamous cell carcinoma.

MeSH terms

Aldehyde Dehydrogenase / metabolism
Animals
Carcinogenesis
Carcinoma, Squamous Cell / physiopathology*
Cell Line, Tumor
Cell Migration Assays
Cell Movement
Cell Survival
Head and Neck Neoplasms / physiopathology*
Humans
Hyaluronan Receptors / metabolism
Mice
Mice, Inbred NOD
Mice, SCID
Neoplasm Transplantation
Neoplastic Stem Cells / metabolism
Neoplastic Stem Cells / physiology*
Radiation Tolerance / physiology*
Squamous Cell Carcinoma of Head and Neck
Wound Healing

Substances

CD44 protein, human
Hyaluronan Receptors
Aldehyde Dehydrogenase