Abstract
Opioid mu-receptors were solubilized from rat brains with 0.5% Triton X-100 in the presence of 0.32 M sucrose and then purified with 6-succinyl morphine-Affi-Gel 102 column. Purified materials showed a major band with mol.wt. 58,000 Da and a minor band with mol.wt. 41,000 Da. Affinity cross linking with [3H]DAGO, a selective mu-opioid agonist, to the neural membranes and purified materials revealed that opioid mu-binding protein has a mol.wt. of 58,000 Da, a value totally different from that of delta- (mol.wt. 18,500 Da) and kappa- (mol.wt. 36,000 Da) binding protein.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain Chemistry*
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Chemical Phenomena
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Chemistry
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Dynorphins / metabolism
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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Enkephalin, D-Penicillamine (2,5)-
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Enkephalins / metabolism
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Ligands
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Male
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Peptide Fragments / metabolism
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Rats
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Rats, Inbred Strains
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Receptors, Opioid / isolation & purification*
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Receptors, Opioid, delta
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Receptors, Opioid, kappa
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Receptors, Opioid, mu
Substances
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Enkephalins
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Ligands
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Peptide Fragments
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Receptors, Opioid
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Receptors, Opioid, delta
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Receptors, Opioid, kappa
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Receptors, Opioid, mu
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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Dynorphins
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Enkephalin, D-Penicillamine (2,5)-