Massively parallel single-nucleus RNA-seq with DroNc-seq

Nat Methods. 2017 Oct;14(10):955-958. doi: 10.1038/nmeth.4407. Epub 2017 Aug 28.


Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human archived brain samples to demonstrate sensitive, efficient, and unbiased classification of cell types, paving the way for systematic charting of cell atlases.

MeSH terms

  • 3T3 Cells
  • Animals
  • Biomarkers
  • HEK293 Cells
  • Humans
  • Mice
  • Principal Component Analysis
  • RNA / genetics*
  • Sequence Analysis, RNA / methods*
  • Single-Cell Analysis / methods
  • Transcription, Genetic


  • Biomarkers
  • RNA