An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues

Nat Methods. 2017 Oct;14(10):959-962. doi: 10.1038/nmeth.4396. Epub 2017 Aug 28.


We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.

MeSH terms

  • Animals
  • Brain
  • Cell Line
  • DNA / genetics*
  • Erythrocytes
  • Freezing*
  • Gene Expression Regulation, Enzymologic
  • Genome*
  • Genome-Wide Association Study
  • Humans
  • Keratinocytes
  • Mice
  • Self-Sustained Sequence Replication
  • Specimen Handling / methods*
  • Thyroid Neoplasms
  • Transposases / metabolism


  • DNA
  • Transposases