Growth, blood health, antioxidant status, immune response and resistance to Aeromonas hydrophila of juvenile yellow catfish exposed to di-2-ethylhexyl phthalate (DEHP)

Comp Biochem Physiol C Toxicol Pharmacol. 2017 Nov;202:79-84. doi: 10.1016/j.cbpc.2017.08.004. Epub 2017 Aug 26.


Triplicate groups of juvenile yellow catfish Pelteobagrus fulvidraco were exposed to three levels of DEHP (0, 0.1 and 0.5mgL-1) for 56days. Fish survival (100%) was not affected by different levels of ambient DEHP. Final body weight, weight gain, specific growth rate and feed intake of fish exposed to 0.5mgL-1 DEHP were the highest. On the contrary, hepatosomatic index of fish exposed to 0.1 and 0.5mgL-1 DEHP were the lowest. Serum total protein, glutamic-pyruvic transaminase, glutamic-oxaloacetic transaminase, glucose and triglycerides increased with the increasing concentrations of DEHP exposure. Superoxide dismutase and glutathione peroxidase activities of fish exposed to 0.5mgL-1 DEHP were the lowest, but malondialdehyde contents of fish exposed to 0.1 and 0.5mgL-1 DEHP were higher than that of control fish. Phagocytic indices of the control group were the highest. After being intraperitoneally injected with Aeromonas hydrophila, fish in the control group had the highest expression of toll like receptor 5, and the expression of myeloid differentiation factor 88 of fish exposed to 0.5mgL-1 DEHP was the lowest. This study indicates that DEHP exerts its toxic effects by interfering with hepatic metabolism, inducing ROS generation and malondialdehyde accumulation, leading to blood deterioration and immunosuppression.

Keywords: Antioxidant; DEHP; Growth; Immune response; Pelteobagrus fulvidraco.

MeSH terms

  • Aeromonas hydrophila*
  • Animals
  • Antioxidants / metabolism*
  • Catfishes / growth & development*
  • Diethylhexyl Phthalate / toxicity*
  • Fish Diseases / immunology
  • Fish Diseases / microbiology
  • Gram-Negative Bacterial Infections / immunology
  • Gram-Negative Bacterial Infections / microbiology
  • Gram-Negative Bacterial Infections / veterinary
  • Immunity, Innate / drug effects*
  • Water Pollutants, Chemical / toxicity


  • Antioxidants
  • Water Pollutants, Chemical
  • Diethylhexyl Phthalate