Diagnosis of Extrapulmonary Legionellosis in Allogeneic Hematopoietic Cell Transplant Recipients by Direct 16S Ribosomal Ribonucleic Acid Sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Open Forum Infect Dis. 2017 Jul 13;4(3):ofx140. doi: 10.1093/ofid/ofx140. eCollection 2017 Summer.

Abstract

Identifying extrapulmonary legionellosis is difficult due to the lack of clinical suspicion and limitations of conventional microbiologic methods. We present a case series of hematopoietic cell transplant recipients with extrapulmonary legionellosis diagnosed via molecular diagnostics: 16S ribosomal ribonucleic acid gene Sanger sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Keywords: 16S ribosomal ribonucleic acid gene sequencing; allogeneic hematopoietic cell transplant; extrapulmonary legionellosis; matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; molecular diagnostics.