Neuronal connections of the central amygdalar nucleus with refeeding-activated brain areas in rats

Brain Struct Funct. 2018 Jan;223(1):391-414. doi: 10.1007/s00429-017-1501-4. Epub 2017 Aug 29.

Abstract

Following fasting, satiety is accompanied by neuronal activation in brain areas including the central amygdalar nucleus (CEA). Since CEA is known to inhibit food intake, we hypothesized that CEA contributes to the termination of meal during refeeding. To better understand the organization of this satiety-related circuit, the interconnections of the CEA with refeeding-activated neuronal groups were elucidated using retrograde (cholera toxin-β subunit, CTB) and anterograde (phaseolus vulgaris leucoagglutinin, PHA-L) tracers in male rats. C-Fos-immunoreactivity was used as marker of neuronal activation. The refeeding-activated input of the CEA primarily originated from the paraventricular thalamic, parasubthalamic and parabrachial nuclei. Few CTB-c-Fos double-labeled neurons were detected in the prefrontal cortex, lateral hypothalamic area, nucleus of the solitary tract (NTS) and the bed nuclei of the stria terminalis (BNST). Only few refeeding-activated proopiomelanocortin-producing neurons of the arcuate nucleus projected to the CEA. Anterograde tract tracing revealed a high density of PHAL-labeled axons contacted with refeeding-activated neurons in the BNST, lateral hypothalamic area, parasubthalamic, paraventricular thalamic and parabrachial nuclei and NTS; a low density of labeled axons was found in the paraventricular hypothalamic nucleus. Chemogenetic activation of the medial CEA (CEAm) inhibited food intake during the first hour of refeeding, while activation of lateral CEA had no effect. These data demonstrate the existence of reciprocal connections between the CEA and distinct refeeding-activated hypothalamic, thalamic and brainstem nuclei, suggesting the importance of short feedback loops in the regulation of satiety and importance of the CEAm in the regulation of food intake during refeeding.

Keywords: Anterograde tract tracing; Connectivity map; Parabrachial nucleus; Parasubthalamic nucleus; Retrograde tract tracing; Satiety; c-Fos.

MeSH terms

  • Analysis of Variance
  • Animals
  • Arcuate Nucleus of Hypothalamus / cytology
  • Arcuate Nucleus of Hypothalamus / physiology
  • Brain Mapping*
  • Central Amygdaloid Nucleus / cytology*
  • Central Amygdaloid Nucleus / physiology*
  • Cholera Toxin / metabolism
  • ELAV-Like Protein 3 / metabolism
  • Eating / physiology
  • Fasting / physiology
  • Feeding Behavior / physiology
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Male
  • Neural Pathways / physiology*
  • Neurons / physiology*
  • Phytohemagglutinins / metabolism
  • Pro-Opiomelanocortin / metabolism
  • Proto-Oncogene Proteins c-fos / metabolism
  • Rats
  • Rats, Wistar
  • Satiety Response / physiology*
  • Transduction, Genetic

Substances

  • ELAV-Like Protein 3
  • Luminescent Proteins
  • Phytohemagglutinins
  • Proto-Oncogene Proteins c-fos
  • leukoagglutinins, plants
  • red fluorescent protein
  • Pro-Opiomelanocortin
  • Cholera Toxin