Tripeptides Restore the Number of Neuronal Spines under Conditions of In Vitro Modeled Alzheimer's Disease

N A Kraskovskaya; E O Kukanova; N S Lin'kova; E A Popugaeva; V Kh Khavinson

doi:10.1007/s10517-017-3847-2

Tripeptides Restore the Number of Neuronal Spines under Conditions of In Vitro Modeled Alzheimer's Disease

Bull Exp Biol Med. 2017 Aug;163(4):550-553. doi: 10.1007/s10517-017-3847-2. Epub 2017 Aug 29.

Authors

N A Kraskovskaya¹, E O Kukanova², N S Lin'kova^{3

4}, E A Popugaeva¹, V Kh Khavinson^{2

5}

Affiliations

¹ Peter the Great Saint Petersburg Polytechnic University, St. Petersburg, Russia.
² St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia.
³ Peter the Great Saint Petersburg Polytechnic University, St. Petersburg, Russia. miayy@yandex.ru.
⁴ St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia. miayy@yandex.ru.
⁵ I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia.

PMID: 28853087
DOI: 10.1007/s10517-017-3847-2

Abstract

In primary culture of mouse hippocampal neurons, peptide EDR (200 ng/ml) under conditions of amyloid synaptotoxicity (a model of Alzheimer's disease) increased the number of mushroom spines by 71% and returned this parameter to the normal level. Under the same conditions, tripeptide KED (200 ng/ml) increased the number of mushroom spines in hippocampal neurons by 20%. Tripeptide EDR can be recommended for further experimental study as a candidate neuroprotective agent for prevention and treatment of Alzheimer's disease.

Keywords: Alzheimer’s disease; culture of hippocampal neurons; dendritic spines; tripeptides; β-amyloid.

MeSH terms

Alzheimer Disease / metabolism*
Amyloid beta-Peptides / metabolism*
Animals
Cells, Cultured
Dendritic Spines / metabolism*
Disease Models, Animal
Hippocampus / cytology*
Mice
Neurons / drug effects*
Peptides / therapeutic use*

Substances

Amyloid beta-Peptides
Peptides