Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha

Elife. 2017 Aug 30;6:e24340. doi: 10.7554/eLife.24340.

Abstract

Cytoplasmic microtubules (cMT) control mitotic spindle positioning in many organisms, and are therefore pivotal for successful cell division. Despite its importance, the temporal control of cMT formation remains poorly understood. Here we show that unlike the best-studied yeast Saccharomyces cerevisiae, position of pre-anaphase nucleus is not strongly biased toward bud neck in Ogataea polymorpha and the regulation of spindle positioning becomes active only shortly before anaphase. This is likely due to the unstable property of cMTs compared to those in S. cerevisiae. Furthermore, we show that cMT nucleation/anchoring is restricted at the level of recruitment of the γ-tubulin complex receptor, Spc72, to spindle pole body (SPB), which is regulated by the polo-like kinase Cdc5. Additionally, electron microscopy revealed that the cytoplasmic side of SPB is structurally different between G1 and anaphase. Thus, polo-like kinase dependent recruitment of γ-tubulin receptor to SPBs determines the timing of spindle orientation in O. polymorpha.

Keywords: Ogataea polymorpha; Spc72; cell biology; cell cycle; cytoplasmic microtubules; infectious disease; microbiology; none; polo-like kinase; spindle pole body.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / metabolism*
  • Microscopy, Electron
  • Microtubule-Associated Proteins / metabolism*
  • Protein-Serine-Threonine Kinases / metabolism*
  • Saccharomycetales / metabolism*
  • Saccharomycetales / ultrastructure
  • Spindle Pole Bodies / metabolism*

Substances

  • Cell Cycle Proteins
  • Microtubule-Associated Proteins
  • Protein-Serine-Threonine Kinases

Grant support

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.