Multiplexed Quantification of Proteins and Transcripts in Single Cells

Nat Biotechnol. 2017 Oct;35(10):936-939. doi: 10.1038/nbt.3973. Epub 2017 Aug 30.

Abstract

We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes and to identify and characterize an unknown cell type.

MeSH terms

  • CD4-Positive T-Lymphocytes / metabolism
  • Humans
  • Lymphocyte Activation / immunology
  • Proteins / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Analysis, Protein
  • Sequence Analysis, RNA
  • Single-Cell Analysis / methods*

Substances

  • Proteins
  • RNA, Messenger