Antioxidant and anti-aging effects of acidic-extractable polysaccharides by Agaricus bisporus

Int J Biol Macromol. 2018 Jan;106:1297-1306. doi: 10.1016/j.ijbiomac.2017.08.135. Epub 2017 Sep 1.


This study was designed to investigate the antioxidant and anti-aging effects of D-galatose-induced (D-gal-induced) aging mice as well as monosaccharide compositions of acidic-extractable polysaccharides (AcAPS) and its major purified fractions (AcAPS-1, AcAPS-2 and AcAPS-3) from the fruiting body of Agaricus bisporus. In the in vitro assays, AcAPS-2 showed superior scavenging activities on hydroxyl (82.98±4.67%) and DPPH (64.47±4.05%) radicals at the concentration of 1.0mg/mL than AcAPS and other polysaccharides fractions. For in vivo anti-aging analysis, AcAPS-2 showed superior effects on hepatic and nephric protection by improving serum enzyme activities, biochemical levels, lipid contents and antioxidant status, respectively. The monosaccharide analysis showed that rhamnose (Rha) and glucose (Glu) may play vital roles in maintaining the antioxidant and anti-aging activities. The results suggested that both AcAPS and its purified fractions might be suitable for functional foods and natural drugs in preventing the acute aging-associated diseases.

Keywords: Acidic-extractable polysaccharides; Antioxidant and anti-aging effects; Purification.

MeSH terms

  • Acids / chemistry
  • Agaricus / chemistry*
  • Aging / drug effects
  • Aging / pathology
  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / chemistry*
  • Biphenyl Compounds / chemistry
  • Free Radical Scavengers / administration & dosage
  • Free Radical Scavengers / chemistry*
  • Fruiting Bodies, Fungal / chemistry
  • Glucose / chemistry
  • Hydroxyl Radical / chemistry
  • Mice
  • Picrates / chemistry
  • Polysaccharides / administration & dosage*
  • Polysaccharides / chemistry
  • Rhamnose / chemistry


  • Acids
  • Antioxidants
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Picrates
  • Polysaccharides
  • Hydroxyl Radical
  • 1,1-diphenyl-2-picrylhydrazyl
  • Glucose
  • Rhamnose