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. 2017 Sep 26;89(13):1330-1337.
doi: 10.1212/WNL.0000000000004412. Epub 2017 Aug 30.

Epstein-Barr Virus, Cytomegalovirus, and Multiple Sclerosis Susceptibility: A Multiethnic Study

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Epstein-Barr Virus, Cytomegalovirus, and Multiple Sclerosis Susceptibility: A Multiethnic Study

Annette Langer-Gould et al. Neurology. .
Free PMC article


Objective: To determine whether Epstein-Barr virus (EBV) or cytomegalovirus (CMV) seropositivity is associated with multiple sclerosis (MS) in blacks and Hispanics and to what extent measures of the hygiene hypothesis or breastfeeding could explain these findings. EBV and CMV have been associated with MS risk in whites, and the timing and frequency of both viruses vary by factors implicated in the hygiene hypothesis.

Methods: Incident cases of MS or its precursor, clinically isolated syndrome (CIS), and matched controls (blacks, 111 cases/128 controls; Hispanics, 173/187; whites, 235/256) were recruited from the membership of Kaiser Permanente Southern California. Logistic regression models accounted for HLA-DRB1*1501 status, smoking, socioeconomic status, age, sex, genetic ancestry, and country of birth.

Results: Epstein-Barr nuclear antigen-1 (EBNA-1) seropositivity was independently associated with an increased odds of MS/CIS in all 3 racial/ethnic groups (p < 0.001 for blacks and whites, p = 0.02 for Hispanics). In contrast, CMV seropositivity was associated with a lower risk of MS/CIS in Hispanics (p = 0.004) but not in blacks (p = 0.95) or whites (p = 0.96). Being born in a low/middle-income country was associated with a lower risk of MS in Hispanics (p = 0.02) but not after accounting for EBNA-1 seropositivity. Accounting for breastfeeding did not diminish the association between CMV and MS in Hispanics.

Conclusions: The consistency of EBNA-1 seropositivity with MS across racial/ethnic groups and between studies points to a strong biological link between EBV infection and MS risk. The association between past CMV infection and MS risk supports the broader hygiene hypothesis, but the inconsistency of this association across racial/ethnic groups implies noncausal associations.


Figure. Association of MS with EBNA-1 and CMV seropositivity among blacks, Hispanics, and whites
Depicted are the adjusted ORs and 95% CIs of the association between (A) EBNA-1 and (B) CMV seropositivity and MS/CIS among blacks (111 cases/128 controls), Hispanics (173 cases/187 controls), and whites (235 cases/256 controls) from the same model. ORs are adjusted for age, sex, education, household income, smoking, HLA-DRB1*15:01 (and HLA-DRB1*15:03 for blacks), genetic ancestry, and (A) CMV or (B) EBNA-1 seropositivity. Country of birth was also included in the model for Hispanics. CI = confidence interval; CIS = clinically isolated syndrome; CMV = cytomegalovirus; EBNA-1 = Epstein-Barr nuclear antigen-1; MS = multiple sclerosis; OR = odds ratio.

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