Adriamycin resistance in HL60 cells in the absence of detectable P-glycoprotein

Biochem Biophys Res Commun. 1987 Jun 30;145(3):1171-6. doi: 10.1016/0006-291x(87)91560-9.


Previous studies have shown that the development of multi-drug resistance in cell lines treated with chemotherapeutic agents is closely associated with the overexpression of a 170-180 kilodalton surface membrane glycoprotein (P-glycoprotein). In the present study a monoclonal antibody against the P-glycoprotein was used to determine if this protein is overexpressed in multi-drug resistant HL60 cells. Using either indirect immunofluorescent staining or immunoblot analysis P-glycoprotein could not be detected in HL60 cells isolated for resistance to adriamycin. In contrast HL60 cells isolated for resistance to vincristine contain the P-glycoprotein and the amount of this material increases with increasing levels of resistance. These studies thus demonstrate adriamycin resistance in P-glycoprotein negative HL60 cells. Furthermore adriamycin and vincristine are found to have distinct effects in inducing overexpression of P-glycoprotein in the HL60 cell line. This information could be useful in the development of therapeutic strategies for the treatment of certain forms of cancer.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Cell Line
  • Cell Membrane / ultrastructure
  • Doxorubicin / pharmacology*
  • Drug Resistance
  • Fluorescent Antibody Technique
  • Glycoproteins / analysis
  • Glycoproteins / genetics*
  • Humans
  • Leukemia, Myeloid, Acute


  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Glycoproteins
  • Doxorubicin