Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Jan;75(1):40-48.
doi: 10.1007/s00284-017-1348-1. Epub 2017 Aug 31.

Extracellular Expression in Aspergillus Niger of an Antibody Fused to Leishmania Sp. Antigens


Extracellular Expression in Aspergillus Niger of an Antibody Fused to Leishmania Sp. Antigens

Denis Magaña-Ortíz et al. Curr Microbiol. .


Nucleoside hydrolase and sterol 24-c-methyltransferase, two antigenic proteins of Leishmania sp., were expressed in Aspergillus niger. Genetic transformation of conidia was achieved using underwater shock waves. scFv antibody addressed to DEC205, a receptor of dendritic cells, was fused to two proteins of Leishmania sp. Receptor 205 has a relevant role in the immune system in mammals; it can modulate T cell response to different antigens. Extracellular expression strategy of recombinant antibody was achieved using a fragment of native glucoamylase A (514 aa) as a carrier. Fermentations in shake flasks showed that the recombinant protein (104 kDa) was expressed and secreted only when maltose was used as carbon source; on the contrary, the expression was highly repressed in presence of xylose. Noteworthy, recombinant protein was secreted without glucoamylase-carrier and accumulation at intracellular level was not observed. The results presented here demonstrate the high value of Aspergillus niger as biotechnological platform for recombinant antibodies against Leishmania sp. at low cost. To the best of our knowledge, this is the first report about the recombinant expression of antigenic proteins of Leishmania sp. in filamentous fungi. The protein obtained can be used to explore novel strategies to induce immunity against Leishmania sp. or it can be employed in diagnostic kits to detect this neglected disease.

Similar articles

See all similar articles

Cited by 3 articles


    1. Curr Genet. 2003 Sep;43(6):439-46 - PubMed
    1. Cell Microbiol. 2015 Sep;17(9):1286-94 - PubMed
    1. Vaccine. 2007 Oct 16;25(42):7450-8 - PubMed
    1. PLoS One. 2013 Jun 26;8(6):e67453 - PubMed
    1. Appl Microbiol Biotechnol. 2005 Jan;66(4):384-92 - PubMed

MeSH terms

LinkOut - more resources