Regulation of Thy-1 gene expression in transgenic mice

Cell. 1987 Jul 31;50(3):445-52. doi: 10.1016/0092-8674(87)90498-3.

Abstract

Genomic DNA fragments encompassing the human Thy-1 or mouse Thy-1.1 gene have been microinjected into pronuclei of mouse embryos homozygous for the Thy-1.2 allele. In the resulting transgenic mice, the human gene is expressed in a pattern characteristic of normal human tissues, and is not influenced by the pattern of endogenous mouse Thy-1 expression. The mouse Thy-1.1 gene fragment is expressed in a pattern typical of mouse Thy-1, although it is more limited in its distribution. The results indicate the presence of multiple cis-acting regulators of Thy-1 gene expression that have changed in both their character and arrangement over the course of Thy-1 gene evolution.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Surface / genetics*
  • DNA, Recombinant*
  • Embryo, Mammalian
  • Gene Expression Regulation*
  • Histocytochemistry
  • Humans
  • Mice
  • Microinjections
  • Thy-1 Antigens

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface
  • DNA, Recombinant
  • Thy-1 Antigens