Window of implantation transcriptomic stratification reveals different endometrial subsignatures associated with live birth and biochemical pregnancy

Fertil Steril. 2017 Oct;108(4):703-710.e3. doi: 10.1016/j.fertnstert.2017.07.007. Epub 2017 Aug 30.


Objective: To refine the endometrial window of implantation (WOI) transcriptomic signature by defining new subsignatures associated to live birth and biochemical pregnancy.

Design: Retrospective cohort study.

Setting: University-affiliated in vitro fertilization clinic and reproductive genetics laboratory.

Patient(s): Healthy fertile oocyte donors (n = 79) and patients with infertility diagnosed by Endometrial Receptivity Analysis (n = 771).

Intervention(s): None.

Main outcome measure(s): WOI transcriptomic signatures associated with specific reproductive outcomes.

Result(s): The retrospective cohort study was designed to perform a prediction model based on transcriptomic clusters for endometrial classification (training set, n = 529). The clinical follow-up set in the expected WOI (n = 321) was tested with the transcriptomic predictor to detect WOI variability and the pregnancy outcomes associated with these subsignatures (n = 228). The endometrial receptivity signature was redefined into four WOI transcriptomic profiles. This stratification identified an optimal endometrial receptivity (RR) signature resulting in an ongoing pregnancy rate (OPR) of 80% in terms of live birth, as well as a late receptive-stage (LR) signature with a potential high risk of 50% biochemical pregnancy. Abnormal down-regulation of the cell cycle was the main dysregulated function among the 22 genes associated with biochemical pregnancy.

Conclusion(s): The major differences between the WOI transcriptomic stratification were in the OPR and biochemical pregnancy rate. The OPR ranged from 76.9% and 80% in the late prereceptive (LPR) and RR signatures, respectively, versus 33.3% in the LR. The biochemical pregnancy rate was 7.7% and 6.6% in LPR and RR, respectively, but 50% in LR, which highlights the relevance of endometrial status in the progression of embryonic implantation.

Keywords: Biochemical pregnancy signature; endometrial genomic medicine; endometrial receptivity; endometrial transcriptomic predictors; transcriptomic stratification of uterine receptivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biomarkers* / blood
  • Biomarkers* / metabolism
  • Embryo Implantation / genetics*
  • Endometrium / metabolism*
  • Female
  • Gene Expression Profiling
  • Humans
  • Live Birth / genetics*
  • Pregnancy
  • Pregnancy Outcome / genetics
  • Pregnancy Rate
  • Retrospective Studies
  • Transcriptome*
  • Young Adult


  • Biomarkers