Liposomes coated with thiolated chitosan as drug carriers of curcumin

Riwang Li; Li Deng; Zhengwei Cai; Shuyun Zhang; Kun Wang; Lihua Li; Shan Ding; Changren Zhou

doi:10.1016/j.msec.2017.05.136

Liposomes coated with thiolated chitosan as drug carriers of curcumin

Mater Sci Eng C Mater Biol Appl. 2017 Nov 1:80:156-164. doi: 10.1016/j.msec.2017.05.136. Epub 2017 May 24.

Authors

Riwang Li¹, Li Deng¹, Zhengwei Cai¹, Shuyun Zhang¹, Kun Wang¹, Lihua Li², Shan Ding¹, Changren Zhou³

Affiliations

¹ Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou, 510632, China.
² Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou, 510632, China. Electronic address: tlihuali@jnu.edu.cn.
³ Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou, 510632, China. Electronic address: tcrz9@jnu.edu.cn.

PMID: 28866151
DOI: 10.1016/j.msec.2017.05.136

Abstract

Liposome is one of a promising delivery system to improve water solubility, stability, and bioavailability of curcumin. But its instability is not favorable for long-circulating treatment, controlled release or conservation. To overcome the disadvantages, thiol derivatised chitosan (CSSH) were synthesized and utilized to coat liposomes. The CSSH coated curcumin liposomes (Cur-Lip-CSSH) had an encapsulation efficiency (EE) of 93.95%, a drug loading (DL) of 7.95%, an average particle size of 406.0nm, and a positive zeta-potential of 36.6mV, which were all higher than that of Cur-Lip. Cur-Lip-CSSH showed slower in vitro release than Cur-Lip at pH5.5 and pH7.4, and the higher retention of curcumin would be remained for the following uptake of cells. The stability of the both liposomes at 4°C was almost the same, but Cur-Lip-CSSH displayed a higher stability at room temperature and higher temperature by DSC characterization. Curcumin can inhibit the growth of cancer cells under certain conditions. MCF-7 cell line was used to study its inhibition and proliferation after treating with curcumin and Cur-Lip-CSSH. Treatment of MCF-7 with curcumin and Cur-Lip-CSSH showed dose and time dependent cytotoxicity, with growth suppression at 200μM, 72h, obviously. These results indicate that the proper coating of liposomes is able to improve the stability of liposomes and the Lip-CSSH can function as potential drug delivery system.

Keywords: Curcumin; Liposome; Stabilization; Thiolated chitosan.

MeSH terms

Chitosan / chemistry*
Curcumin
Drug Carriers
Humans
Liposomes
MCF-7 Cells
Particle Size

Substances

Drug Carriers
Liposomes
Chitosan
Curcumin