Identification of an argpyrimidine-modified protein in human red blood cells from schizophrenic patients: A possible biomarker for diseases involving carbonyl stress

Biochem Biophys Res Commun. 2017 Nov 4;493(1):573-577. doi: 10.1016/j.bbrc.2017.08.150. Epub 2017 Sep 1.

Abstract

Argpyrimidine (ARP) is an advanced glycation end product thought to be generated from a reaction between methylglyoxal and arginine residues in proteins. In this study, we observed marked accumulation of an approximately 56 kD protein, reactive to anti-ARP antibodies, in the red blood cells (RBCs) of some patients with refractory schizophrenia. This ARP-modified protein was purified from the blood of schizophrenic patients and identified as selenium binding protein 1 (SBP1) by LC-MS/MS. This is the first report of ARP-modified proteins accumulating in RBCs of patients with diseases involving carbonyl stress. We also observed high accumulation of ARP-modified SBP1 in the RBCs of patients with chronic kidney disease. Therefore, this modified protein may be a novel marker of carbonyl stress.

Keywords: Argpyrimidine; Biomarker; Carbonyl stress; Red blood cell; Schizophrenia; Selenium binding protein 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers
  • Erythrocytes / metabolism*
  • Female
  • Humans
  • Japan / epidemiology
  • Male
  • Ornithine / analogs & derivatives*
  • Ornithine / blood
  • Prevalence
  • Protein Carbonylation*
  • Pyrimidines / blood*
  • Reproducibility of Results
  • Risk Assessment
  • Schizophrenia / blood*
  • Schizophrenia / diagnosis
  • Schizophrenia / epidemiology*
  • Selenium-Binding Proteins / blood*
  • Sensitivity and Specificity

Substances

  • Biomarkers
  • Pyrimidines
  • SELENBP1 protein, human
  • Selenium-Binding Proteins
  • argpyrimidine
  • Ornithine