Borrelia burgdorferi basic membrane protein A could induce chemokine production in murine microglia cell line BV2

Microb Pathog. 2017 Oct;111:174-181. doi: 10.1016/j.micpath.2017.08.036. Epub 2017 Sep 1.

Abstract

Lyme neuroborreliosis is a nervous system infectious disease caused by Borrelia burgdorferi (B. burgdorferi). It has been demonstrated that cytokines induced by B. burgdorferi are related to Lyme neuroborreliosis. Microglia is known as a key player in the immune responses that occur within the central nervous system. In response to inflammation, it will be activated and generate cytokines and chemokines. Experiments in vitro cells have showed that B. Burgdorferi membrane protein A (BmpA), a major immunogen of B. Burgdorferi, could induce Lyme arthritis and stimulate human and murine lymphocytes to produce inflammatory cytokines. In our study, the murine microglia BV2 cell line was used as a cell model to explore the stimulating effects of recombinant BmpA (rBmpA); Chemokine chip, ELISA and QPCR technology were used to measure the production of chemokines from microglial cells stimulated by rBmpA. Compared with the negative control group, CXCL2, CCL22, and CCL5 concentrations in the cell supernatant increased significantly after the rBmpA stimulation; the concentration of these chemokines increased with rBmpA concentration increasing; the mRNA expression levels of chemokines (CXCL2, CCL22, and CCL5) in murine BV2 cells increased significantly with 10 μg/mL and 20 μg/mL rBmpA stimulation; CXCL13 was not change after the rBmpA stimulation. Our study shows that chemokines, such as CXCL2, CCL22, and CCL5 were up-regulated by the rBmpA in the BV2 cells. The production of chemokines in Lyme neuroborreliosis may be mainly from microglia cells and the rBmpA may be closely related with the development of Lyme neuroborreliosis.

Keywords: Borrelia burgdorferi; Chemokine; Lyme neuroborreliosis; Microglia cell; rBmpA.

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Borrelia burgdorferi / immunology*
  • Borrelia burgdorferi / metabolism*
  • Cell Line / drug effects
  • Chemokine CCL22 / metabolism
  • Chemokine CCL5 / metabolism
  • Chemokine CXCL2 / metabolism
  • Chemokines / metabolism*
  • Cytokines / metabolism
  • Genes, Bacterial / genetics
  • Humans
  • Inflammation / immunology
  • Lyme Disease / immunology
  • Lyme Neuroborreliosis / immunology*
  • Lymphocytes / immunology
  • Membrane Proteins / immunology*
  • Mice
  • Microglia / immunology*
  • Recombinant Proteins
  • Staphylococcal Protein A

Substances

  • Bacterial Proteins
  • Ccl22 protein, mouse
  • Ccl5 protein, mouse
  • Chemokine CCL22
  • Chemokine CCL5
  • Chemokine CXCL2
  • Chemokines
  • Cxcl2 protein, mouse
  • Cytokines
  • Membrane Proteins
  • P39 antigen, Borrelia burgdorferi
  • Recombinant Proteins
  • Staphylococcal Protein A