Antioxidant and glucose metabolizing potential of edible insect, Brachytrupes orientalis via modulating Nrf2/AMPK/GLUT4 signaling pathway

Prachurjya Dutta; Tapan Dey; Anjum Dihingia; Prasenjit Manna; Jatin Kalita

doi:10.1016/j.biopha.2017.08.094

Antioxidant and glucose metabolizing potential of edible insect, Brachytrupes orientalis via modulating Nrf2/AMPK/GLUT4 signaling pathway

Biomed Pharmacother. 2017 Nov:95:556-563. doi: 10.1016/j.biopha.2017.08.094. Epub 2017 Sep 12.

Authors

Prachurjya Dutta¹, Tapan Dey², Anjum Dihingia¹, Prasenjit Manna¹, Jatin Kalita³

Affiliations

¹ Biological Science and Technology Division, CSIR-North East Institute of Science and Technology (CSIR-NEIST), Jorhat- 785006, Assam, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-North East Institute of Science and Technology (CSIR-NEIST) campus, Jorhat.
² Biological Science and Technology Division, CSIR-North East Institute of Science and Technology (CSIR-NEIST), Jorhat- 785006, Assam, India; Centre for Biotechnology and Bioinformatics, Dibrugarh University, Dibrugarh-786004, Assam, India.
³ Biological Science and Technology Division, CSIR-North East Institute of Science and Technology (CSIR-NEIST), Jorhat- 785006, Assam, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-North East Institute of Science and Technology (CSIR-NEIST) campus, Jorhat. Electronic address: kalitajk74@gmail.com.

PMID: 28869893
DOI: 10.1016/j.biopha.2017.08.094

Abstract

Brachytrupes orientalis (Gryllidae) is a common edible insect species eaten by the different tribes of North East India. This study investigated the potentiality of Brachytrupes orientalis extracts in different solvent hydro-alcoholic (AEBO), hexane (HEBO) and ethyl acetate (EEBO) on glucose utilization and cell viability in high glucose (HG) treated myotubes. It has been observed that AEBO supplementation significantly increased the glucose utilization against HG exposure; however, treatment HEBO and EEBO have no significant effect. AEBO also increased the intercellular glucose-6-phosphate level and the protein expression of both phospho-AMPK and GLUT4 in HG treated myotubes in a dose dependent manner. Furthermore, supplementation with AEBO decreased the intercellular ROS production, lipid peroxidation, and up-regulated the protein expression of Nrf2 and GST. Chromatography and Spectroscopic analyses of AEBO also suggest that Ursolic acid may be one of the bioactive principles with rich potassium, sodium, calcium and magnesium content.

Keywords: Antioxidant; Brachytrupes orientalis; Edible insect; Entomotherapeutics; Glucose utilization.

MeSH terms

Adenylate Kinase / metabolism
Animals
Antioxidants / pharmacology*
Cell Line
Cell Survival / drug effects
Cell-Free System
Free Radical Scavengers / metabolism
Glucose / metabolism*
Glucose Transporter Type 4 / metabolism*
Glucose-6-Phosphate / metabolism
Insecta / physiology*
Intracellular Space / metabolism
Lipid Peroxidation / drug effects
Malondialdehyde / metabolism
Mice
Muscle Fibers, Skeletal / drug effects
Muscle Fibers, Skeletal / metabolism
NF-E2-Related Factor 2 / metabolism*
Oxidative Stress / drug effects
Phosphorylation / drug effects
Reactive Oxygen Species / metabolism
Signal Transduction / drug effects*
Solvents
Spectroscopy, Fourier Transform Infrared

Substances

Antioxidants
Free Radical Scavengers
Glucose Transporter Type 4
NF-E2-Related Factor 2
Reactive Oxygen Species
Solvents
Malondialdehyde
Glucose-6-Phosphate
Adenylate Kinase
Glucose