Effect of 1-y oral supplementation with vitaminized olive oil on platelets from healthy postmenopausal women

Nutrition. 2017 Oct:42:92-98. doi: 10.1016/j.nut.2017.06.013. Epub 2017 Jul 5.


Objective: Olive oil is the main fat source in the Mediterranean diet and shows a protective role against aging and related diseases. Osteoporosis represents a serious health problem worldwide and is associated with an increased risk for fractures and mortality. Nutrition should be part of bone disease prevention strategies, especially in light of the aging population and the effect of diet on bone health. The aim of this study was to investigate whether oral supplementation with extra virgin olive oil (VOO) enriched with vitamins D3, K1, and B6 (VitVOO) is able to modify some physicochemical and functional plasma membrane properties and nitrosative stress markers status.

Methods: In this single-center, randomized placebo-controlled trial, 60 postmenopausal women were administered either VitVOO or placebo (PlaVOO). After 1 y of oral supplementation, platelet membrane fluidity changes, Na+/K+-ATPase activity, serum nitric oxide, and peroxynitrite levels were determined in participants.

Results: After 1 y (time 1), women taking VitVOO showed lower nitric oxide levels than those taking PlaVOO; the same trend was found for peroxynitrite levels. As far as membrane fluidity was concerned, a significant decrease in anisotropy of diphenylhexatriene and trimethylammonium-diphenylhexatriene at time 1 in VitVOO participants compared with PlaVOO was found. Finally, Na+/K+-ATPase activity showed a significant increase after VitVOO supplementation.

Conclusion: The supplementation of VitVOO into the diet of postmenopausal women could represent a proper tool for platelet function and a useful strategy against nitrosative stress and related diseases, thus confirming the antioxidant role played by the added vitamins.

Keywords: Membrane fluidity; Menopause; Na(+)/K(+)-ATPase activity; Nitrosative stress; Platelet; Vitaminized oil.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Blood Platelets / drug effects*
  • Cholecalciferol / therapeutic use
  • Dietary Supplements*
  • Female
  • Humans
  • Middle Aged
  • Nitric Oxide / biosynthesis
  • Nitrosative Stress / drug effects
  • Olive Oil / therapeutic use*
  • Oxidative Stress / drug effects
  • Peroxynitrous Acid / blood
  • Postmenopause*
  • Vitamin B 6 / therapeutic use
  • Vitamin K 1 / therapeutic use
  • Vitamins / therapeutic use*


  • Olive Oil
  • Vitamins
  • Peroxynitrous Acid
  • Cholecalciferol
  • Nitric Oxide
  • Vitamin B 6
  • Vitamin K 1