Sea Buckthorn (Hippophaë rhamnoides L.) Oil Improves Atopic Dermatitis-Like Skin Lesions via Inhibition of NF-κB and STAT1 Activation

Skin Pharmacol Physiol. 2017;30(5):268-276. doi: 10.1159/000479528. Epub 2017 Sep 6.


Background and objectives: The objective of this study was to evaluate the topical effects of sea buckthorn (SBT) oil on atopic dermatitis (AD)-like lesions in a mouse model generated by repeated topical administration of DNCB in BALB/c mice.

Methods: DNCB was applied repeatedly on the dorsal skin of mice to induce AD-like lesions. Following AD induction, SBT oil was applied daily on the dorsal skin for 4 weeks. The severity of skin lesions was examined macroscopically and histologically. We further measured the production of MDC/CCL22 and TARC/CCL17 in IFN-γ/TNF-α activated HaCaT cells.

Results: Topically applied SBT oil in DNCB-treated mice ameliorated the severity score of dermatitis, decreased epidermal thickness, reduced spleen and lymph node weights, and prevented mast cell infiltration. In addition, SBT oil suppressed the Th2 chemokines TARC and MDC via dose-dependent inhibition of NF-κB, JAK2/STAT1, and p38-MAPK signaling pathways in IFN-γ/TNF-α-activated HaCaT cells.

Conclusion: These results suggest that SBT oil had a beneficial effect on AD-like skin lesions, partially via inhibition of the Th2 chemokines TARC and MDC in inflamed skin.

Keywords: Atopic dermatitis; DNCB; Hippophaë rhamnoides L.; NF-κB; STAT1; Sea buckthorn oil.

MeSH terms

  • Animals
  • Cell Line
  • Chemokine CCL17 / metabolism
  • Chemokine CCL22 / metabolism
  • Dermatitis, Atopic / drug therapy*
  • Dermatitis, Atopic / metabolism
  • Dermatitis, Atopic / pathology
  • Dinitrochlorobenzene
  • Female
  • Hippophae*
  • Humans
  • Irritants
  • Lymph Nodes / drug effects
  • Mice, Inbred BALB C
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / metabolism
  • Plant Oils / pharmacology
  • Plant Oils / therapeutic use*
  • STAT1 Transcription Factor / antagonists & inhibitors
  • STAT1 Transcription Factor / metabolism
  • Skin / drug effects
  • Skin / metabolism
  • Skin / pathology
  • Spleen / drug effects


  • Ccl17 protein, mouse
  • Ccl22 protein, mouse
  • Chemokine CCL17
  • Chemokine CCL22
  • Dinitrochlorobenzene
  • Irritants
  • NF-kappa B
  • Plant Oils
  • STAT1 Transcription Factor
  • Stat1 protein, mouse