CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Sci Rep. 2017 Sep 5;7(1):10487. doi: 10.1038/s41598-017-08596-5.

Abstract

Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Genetic Therapy / methods
  • Genetic Therapy / veterinary*
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Humans
  • Organogenesis / genetics*
  • Pancreas / embryology
  • Pancreas / metabolism*
  • Pluripotent Stem Cells / metabolism
  • Pluripotent Stem Cells / transplantation
  • Stem Cell Transplantation / methods
  • Stem Cell Transplantation / veterinary
  • Swine / embryology
  • Swine / genetics*
  • Tissue and Organ Harvesting / methods
  • Tissue and Organ Harvesting / veterinary
  • Trans-Activators / genetics
  • Trans-Activators / metabolism

Substances

  • Homeodomain Proteins
  • Trans-Activators
  • pancreatic and duodenal homeobox 1 protein