Morphologic and physiologic studies of canine ileal enteroglucagon-containing cells in short-term culture

Gastroenterology. 1987 Oct;93(4):791-800. doi: 10.1016/0016-5085(87)90442-2.


Enteroglucagon-containing cells have been maintained in short-term culture, and the morphologic characteristics of these cells and their response to selected agents have been determined. After 48 h in culture the ultrastructural appearance of the enteroglucagon-immunoreactive cells showed evidence of polarization with re-formation of apical microvilli and the secretory granules concentrated at the opposite pole of the cell. The size of the intracellular secretory granules was 370 +/- 15 nm. The release of enteroglucagonlike immunoreactivity was stimulated in a dose-dependent manner by the adrenergic agonists epinephrine and isoproterenol. The response to epinephrine was competitively inhibited by propranolol, producing a rightward shift of the dose-responsive curve. The alpha-adrenergic agonists methoxamine and clonidine did not stimulate enteroglucagon release above basal. The adenyl cyclase activator forskolin also stimulated release of the peptide in a dose-dependent manner. Carbachol and somatostatin produced a dose-dependent inhibition of epinephrine-stimulated release, indicating direct inhibitory modulation of enteroglucagonlike immunoreactive cells. Somatostatin also inhibited forskolin-stimulated release. These data indicate that canine ileal enteroglucagon cells in short-term culture respond to a number of specific stimuli.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Separation
  • Cells, Cultured
  • Chromatography, Gel
  • Colforsin / antagonists & inhibitors
  • Colforsin / pharmacology
  • Cytoplasmic Granules / metabolism
  • Dogs
  • Epinephrine / antagonists & inhibitors
  • Epinephrine / pharmacology
  • Gastrointestinal Hormones / metabolism*
  • Glucagon-Like Peptides / metabolism*
  • Histocytochemistry
  • Ileum / drug effects
  • Ileum / metabolism*
  • Ileum / ultrastructure
  • Immunologic Techniques
  • Somatostatin / pharmacology


  • Gastrointestinal Hormones
  • Colforsin
  • Somatostatin
  • Glucagon-Like Peptides
  • Epinephrine