[Effects of Tongluo Xingnao effervescent tablets on blood rheology, iNOS, VEGF and LDH-5 in MID rats]

Zhongguo Zhong Yao Za Zhi. 2016 Mar;41(6):1119-1123. doi: 10.4268/cjcmm20160624.
[Article in Chinese]


The study was to explore effects of Tongluo Xingnao effervescent tablets on the blood rheology, iNOS, VEGF and LDH-5 in multi-infarct dementia(MID) model rats. Establish MID model rats were induced by microthrombosis, from which 50 successful model rats were randomly divided into five groups, such as the model control group, the dihydroergotoxine mesylate tablets(hydergine) group(0.7 mg•kg⁻¹), Tongluo Xingnao effervescent tablets high-dose, medium-dose and low-dose groups(7.56, 3.78, 1.89 g•kg⁻¹). Another ten rats in the sham group were randomly selected as the parallel control group. Each group was orally administered with drugs for 90 days. The learning and memory ability was evaluated with the Morris water maze test, while the whole blood viscosity and the erythrocyte aggregation index derived from abdominal aorta were measured in different shear rates. In addition, the levels of VEGF and iNOS in the serum were determined by ELISA kits. The expression of LDH-5 in hippocampus of rats was measured with immunohistochemistry and image quantitative analysis. The result showed that Tongluo Xingnao effervescent tablets notably decreased the escape latency of MID model rats, increased times of entering into the escape platform and prolonged retention time in medium ring, meanwhile the whole blood viscosity in MID model rats was also notably reduced in four shear rates, i.e. 1, 5, 30, 200 S⁻¹, erythrocyte aggregation index, serum VEGF and iNOS, and average optical density value of LDH-5, with a statistically significant differences compared with the model control group (P<0.05). In conclusion, Tongluo Xingnao effervescent tablets could improve the ability of learning and memory of MID model rats and the blood rheology, reduce the level of iNOS, VEGF and the expression of LDH-5, and then improved the brain energy supply.

Keywords: LDH-5; Tongluo Xingnao effervescent tablets; VEGF; blood rheology; iNOS; multi-infarct dementia.

MeSH terms

  • Animals
  • Blood Chemical Analysis*
  • Dementia, Multi-Infarct / blood
  • Dementia, Multi-Infarct / drug therapy*
  • Dementia, Multi-Infarct / metabolism
  • Dementia, Multi-Infarct / psychology
  • Drugs, Chinese Herbal / administration & dosage*
  • Hippocampus / drug effects
  • Hippocampus / metabolism
  • Humans
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • L-Lactate Dehydrogenase / genetics
  • L-Lactate Dehydrogenase / metabolism*
  • Lactate Dehydrogenase 5
  • Learning / drug effects
  • Male
  • Memory / drug effects
  • Nitric Oxide Synthase Type II / genetics
  • Nitric Oxide Synthase Type II / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Rheology
  • Tablets / administration & dosage
  • Vascular Endothelial Growth Factor A / genetics
  • Vascular Endothelial Growth Factor A / metabolism*


  • Drugs, Chinese Herbal
  • Isoenzymes
  • Tablets
  • Vascular Endothelial Growth Factor A
  • tongluo
  • L-Lactate Dehydrogenase
  • Lactate Dehydrogenase 5
  • Nitric Oxide Synthase Type II
  • Nos2 protein, rat