Effect of Vegetable Oils Applied Over Acquired Enamel Pellicle on Initial Erosion

J Appl Oral Sci. Jul-Aug 2017;25(4):420-426. doi: 10.1590/1678-7757-2016-0436.


Objective: The prevalence of dental erosion has been recently increasing, requiring new preventive and therapeutic approaches. Vegetable oils have been studied in preventive dentistry because they come from a natural, edible, low-cost, and worldwide accessible source. This study aimed to evaluate the protective effect of different vegetable oils, applied in two concentrations, on initial enamel erosion.

Material and methods: Initially, the acquired pellicle was formed in situ for 2 hours. Subsequently, the enamel blocks were treated in vitro according to the study group (n=12/per group): GP5 and GP100 - 5% and pure palm oil, respectively; GC5 and GC100 - 5% and pure coconut oil; GSa5 and GSa100 - 5% and pure safflower oil; GSu5 and GSu100 - 5% and pure sunflower oil; GO5 and GO100 - 5% and pure olive oil; CON- - Deionized Water (negative control) and CON+ - Commercial Mouthwash (Elmex® Erosion Protection Dental Rinse, GABA/positive control). Then, the enamel blocks were immersed in artificial saliva for 2 minutes and subjected to short-term acid exposure in 0.5% citric acid, pH 2.4, for 30 seconds, to promote enamel surface softening. The response variable was the percentage of surface hardness loss [((SHi - SHf) / SHf )×100]. Data were analyzed by one-way ANOVA and Tukey's test (p<0.05).

Results: Enamel blocks of GP100 presented similar hardness loss to GSu100 (p>0.05) and less than the other groups (p<0.05). There was no difference between GP5, GC5, GC100, GSa5, GSu100, GSa100, GSu5, GO5, GO100, CON- and CON+.

Conclusion: Palm oil seems to be a promising alternative for preventing enamel erosion. However, further studies are necessary to evaluate a long-term erosive cycling.

Publication types

  • Evaluation Study

MeSH terms

  • Dental Pellicle / drug effects*
  • Hardness Tests
  • Humans
  • Materials Testing
  • Palm Oil
  • Plant Oils / pharmacology
  • Plant Oils / therapeutic use*
  • Random Allocation
  • Reproducibility of Results
  • Saliva / chemistry
  • Saliva, Artificial
  • Surface Properties
  • Time Factors
  • Tooth Demineralization / prevention & control
  • Tooth Erosion / prevention & control*
  • Treatment Outcome
  • Young Adult


  • Plant Oils
  • Saliva, Artificial
  • Palm Oil