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. 2017 Dec;32(6):545-547.
doi: 10.1007/s12250-017-4057-9.

Antiviral Activity of quercetin-3-β-O-D-glucoside Against Zika Virus Infection

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Free PMC article

Antiviral Activity of quercetin-3-β-O-D-glucoside Against Zika Virus Infection

Gary Wong et al. Virol Sin. .
Free PMC article

Abstract

Q3G is a natural derivative of quercetin and is already widely used in various foods and drinks. Our results clearly demonstrated that Q3G exerts antiviral activity against ZIKV in both tissue culture and knockout mice, and that post-exposure in vivo treatment with Q3G could have a beneficial effect. In the future, Q3G should be tested in human cell lines (such as Huh-7, HeLa, or K048, a fetal brain neural stem cell line) to provide further data supporting its potential efficacy in humans; in addition, live viral loads or viremia should be tested in treated animals to supplement the survival results observed in this study. Although the treatment regimens will need to be further optimized (i.e., dosage, frequency of treatment, and administration routes), our results support the results of Q3G efficacy studies in nonhuman primates against ZIKV infection. Further studies will also be needed to investigate the mechanism of Q3G antiviral action, in order to obtain valuable insights into the design of novel targets for antiviral therapeutics in the future.

Figures

Figure 1
Figure 1
Antiviral activity of Q3G against ZIKV in cell culture and treatment of ZIKV-infected Ifnar1−/− mice with Q3G. Viral inhibition curves of various concentrations of Q3G against ZIKV PLCal_ZV assessed at (A) 2 days post-infection (pink circles) or 4 days post-infection (blue triangles). Quantification of ZIKV NS1 expression by ELISA from the (B) supernatant and (C) cell lysate of Vero cells infected with PLCal_ZV and treated with various concentrations of Q3G. “Mock” indicates mock infection. All experiments were performed in triplicate, and error bars represent mean ± standard deviation. ** P < 0.01; *** P < 0.001. (D) Post-exposure treatment schedule. (E) Survival and (F) weight change over time. Mice (n = 6/group) were treated with either PBS (pink circles) or Q3G (green triangles) and challenged intraperitoneally with 1 × 106 PFU of the PRVABC59 isolate. Error bars indicate standard error of the mean.

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