Altered synaptobrevin-II Trafficking in Neurons Expressing a Synaptophysin Mutation Associated With a Severe Neurodevelopmental Disorder

Neurobiol Dis. 2017 Dec;108:298-306. doi: 10.1016/j.nbd.2017.08.021. Epub 2017 Sep 5.

Abstract

Following exocytosis, synaptic vesicles (SVs) have to be reformed with the correct complement of proteins in the correct stoichiometry to ensure continued neurotransmission. Synaptophysin is a highly abundant, integral SV protein necessary for the efficient retrieval of the SV SNARE protein, synaptobrevin II (sybII). However the molecular mechanism underpinning synaptophysin-dependent sybII retrieval is still unclear. We recently identified a male patient with severe intellectual disability, hypotonia, epilepsy and callosal agenesis who has a point mutation in the juxtamembrane region of the fourth transmembrane domain of synaptophysin (T198I). This mutation had no effect on the activity-dependent retrieval of synaptophysin that was tagged with the genetically-encoded pH-sensitive reporter (pHluorin) in synaptophysin knockout hippocampal cultures. This suggested the mutant has no global effect on SV endocytosis, which was confirmed when retrieval of a different SV cargo (the glutamate transporter vGLUT1) was examined. However neurons expressing this T198I mutant did display impaired activity-dependent sybII retrieval, similar to that observed in synaptophysin knockout neurons. Interestingly this impairment did not result in an increased stranding of sybII at the plasma membrane. Screening of known human synaptophysin mutations revealed a similar presynaptic phenotype between T198I and a mutation found in X-linked intellectual disability. Thus this novel human synaptophysin mutation has revealed that aberrant retrieval and increased plasma membrane localisation of SV cargo can be decoupled in human disease.

Keywords: Endocytosis; Nerve terminal; Neuron; Synaptic vesicle; Synaptobrevin; Synaptophysin.

Publication types

  • Case Reports

MeSH terms

  • Animals
  • Biological Transport, Active / physiology*
  • Cell Membrane / metabolism
  • Cell Membrane / pathology
  • Cells, Cultured
  • Child
  • Developmental Disabilities / genetics
  • Developmental Disabilities / metabolism*
  • Endocytosis / physiology
  • Female
  • Hippocampus / metabolism
  • Hippocampus / pathology
  • Humans
  • Male
  • Mental Retardation, X-Linked / genetics
  • Mental Retardation, X-Linked / metabolism
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mutation
  • Neurons / metabolism*
  • Neurons / pathology
  • Synaptic Vesicles / metabolism
  • Synaptic Vesicles / pathology
  • Synaptophysin / deficiency
  • Synaptophysin / genetics
  • Vesicle-Associated Membrane Protein 2 / metabolism*

Substances

  • Synaptophysin
  • Syp protein, mouse
  • Vesicle-Associated Membrane Protein 2