Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures

Vaccine. 2017 Oct 9;35(42):5576-5580. doi: 10.1016/j.vaccine.2017.08.053. Epub 2017 Sep 6.


Analysis of the anti-haemagglutinin serum antibody proteome from six H1N1pdm09 influenza A vaccinated subjects demonstrated restricted IgG1 heavy chain species encoded by IGHV5-51 and IGHV3-7 gene families in 2 subjects and either IGHV5-51 or IGHV3-7 in 4 individuals. All subjects exhibited a dominant IGKV3-20 light chain, however 5 subjects also exhibited IGKV3-11 and IGKV4-1 families. Sequences were closely aligned with the matched germline sequence, with few shared mutations. This study illustrates the feasibility of using a proteomic approach to determine the expressed V region signatures of serum antibodies induced by vaccination.

Keywords: Influenza A; Mass spectrometric sequencing; Proteomics; Serum antibody repertoire; Vaccination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Amino Acid Sequence
  • Antibodies, Viral / immunology*
  • Female
  • Hemagglutinins / immunology*
  • Humans
  • Immunoglobulin Variable Region / immunology*
  • Influenza, Human / immunology*
  • Influenza, Human / prevention & control*
  • Male
  • Middle Aged
  • Proteome / immunology*
  • Proteomics / methods
  • Vaccination / methods


  • Antibodies, Viral
  • Hemagglutinins
  • Immunoglobulin Variable Region
  • Proteome