Comparison of three multiplex gastrointestinal platforms for the detection of gastroenteritis viruses

J Clin Virol. 2017 Oct;95:66-71. doi: 10.1016/j.jcv.2017.08.012. Epub 2017 Sep 1.

Abstract

Background: Viruses are major etiological agents of childhood gastroenteritis. In recent years, several molecular platforms for the detection of viral enteric pathogens have become available.

Objective/study design: We evaluated the performance of three multiplex platforms including Biofire's Gastrointestinal Panel (FilmArray), Luminex xTAG® Gastrointestinal Pathogen Panel (GPP), and the TaqMan Array Card (TAC) for the detection of five gastroenteritis viruses using a coded panel of 300 archived stool samples.

Results: The FilmArray detected a virus in 199 (96.1%) and the TAC in 172 (83.1%) of the 207 samples (187 samples positive for a single virus and 20 samples positive for more than one virus) whereas the GPP detected a virus in 100 (78.7%) of the 127 (97 positive for one virus and three positive for more than one virus) samples. Overall the clinical accuracy was highest for the FilmArray (98%) followed by TAC (97.2%) and GPP (96.9%). The sensitivity of the FilmArray, GPP and TAC platforms was highest for rotavirus (100%, 95.8%, and 89.6%, respectively) and lowest for adenovirus type 40/41 (97.4%, 57.9% and 68.4%). The specificity of the three platforms ranged from 95.6% (rotavirus) to 99.6% (norovirus/sapovirus) for the FilmArray, 99.6% (norovirus) to 100% (rotavirus/adenovirus) for GPP, and 98.9% (astrovirus) to 100% (rotavirus/sapovirus) for TAC.

Conclusion: The FilmArray demonstrated the best analytical performance followed by TAC. In recent years, the availability of multi-enteric molecular testing platforms has increased significantly and our data highlight the strengths and weaknesses of these platforms.

Keywords: Adenovirus type 40/41; Astrovirus; Gastroenteritis viruses; Norovirus; Rotavirus; Sapovirus.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Feces / virology
  • Gastroenteritis / diagnosis*
  • Gastroenteritis / virology*
  • Humans
  • Molecular Diagnostic Techniques / instrumentation
  • Molecular Diagnostic Techniques / methods
  • Multiplex Polymerase Chain Reaction / instrumentation
  • Multiplex Polymerase Chain Reaction / methods*
  • Norovirus / genetics
  • Norovirus / isolation & purification*
  • Rotavirus / genetics
  • Rotavirus / isolation & purification*
  • Sapovirus / genetics
  • Sapovirus / isolation & purification*
  • Sensitivity and Specificity
  • Virus Diseases / diagnosis*
  • Virus Diseases / virology