CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation

Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11.


Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.

MeSH terms

  • CRISPR-Cas Systems*
  • Cell Differentiation
  • Cells, Cultured
  • DNA, Complementary
  • Fibroblasts
  • Gene Expression Regulation / physiology
  • Humans
  • Light
  • Luminescent Measurements
  • Neurons / physiology*
  • Optogenetics / methods*
  • Transcription, Genetic


  • DNA, Complementary