Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Aug 28;10:4225-4238.
doi: 10.2147/OTT.S137107. eCollection 2017.

miRNA Expression Profiling in Formalin-Fixed Paraffin-Embedded Endometriosis and Ovarian Cancer Samples

Affiliations
Free PMC article

miRNA Expression Profiling in Formalin-Fixed Paraffin-Embedded Endometriosis and Ovarian Cancer Samples

Ovidiu-Leonard Braicu et al. Onco Targets Ther. .
Free PMC article

Abstract

Endometriosis is an inflammatory pathology associated with a negative effect on life quality. Recently, this pathology was connected to ovarian cancer, in particular with endometrioid ovarian cancer. microRNAs (miRNAs) are a class of RNA transcripts ~19-22 nucleotides in length, the altered miRNA pattern being connected to pathological status. miRNAs are highly stable transcripts, and these can be assessed from formalin-fixed paraffin-embedded (FFPE) samples leading to the identification of miRNAs that could be developed as diagnostic and prognostic biomarkers, in particular those involved in malignant transformation. The aim of our study was to evaluate miRNA expression pattern in FFPE samples from endometriosis and ovarian cancer patients using PCR-array technology and also to compare the differential expression pattern in ovarian cancer versus endometriosis. For the PCR-array study, we have used nine macrodissected FFPE samples from endometriosis tissue, eight samples of ovarian cancers and five normal ovarian tissues. Quantitative real-time PCR (qRT-PCR) was used for data validation in a new patient cohort of 17 normal samples, 33 endometriosis samples and 28 ovarian cancer macrodissected FFPE samples. Considering 1.5-fold expression difference as a cut-off level and a P-value <0.05, we have identified four miRNAs being overexpressed in endometrial tissue, while in ovarian cancer 15 were differentially expressed (nine overexpressed and six downregulated). The expression level was confirmed by qRT-PCR for miR-93, miR-141, miR-155, miR-429, miR-200c, miR-205 and miR-492. Using the interpretative program Ingenuity Pathway Analysis revealed several deregulated pathways due to abnormal miRNA expression in endometriosis and ovarian cancer, which in turn is responsible for pathogenesis; this differential expression of miRNAs can be exploited as a therapeutic target. A higher number of altered miRNAs were detected in endometriosis versus ovarian cancer tissue, most of them being linked with epithelial-to-mesenchymal transition.

Keywords: endometriosis; formalin-fixed paraffin-embedded samples; miRNA; ovarian cancer.

Conflict of interest statement

Disclosure The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Venn diagram presenting the altered expression levels of common and differentially expressed miRNAs in the analyzed subgroups.
Figure 2
Figure 2
Network generated for the altered miRNAs in the analysis of endometriosis versus normal tissue. The upregulated miRNAs are displayed in red.
Figure 3
Figure 3
Network generated for the altered miRNAs in the analysis of ovarian cancer versus control. The upregulated miRNAs are displayed in red and the downregulated miRNAs in green.
Figure 4
Figure 4
qRT-PCR validation in endometriosis and ovarian cancer patient cohort for miR-93, miR-141, miR-155, miR-429, miR-200c, miR-205 and miR-492. ROC curve analysis of expression levels of miRNAs for endometriosis and ovarian cancer group. The figure displays AUC for each evaluated miRNA, a parameter that indicates the precision in discriminating the endometrial tissue and ovarian cancer tissue from the normal tissue. *P<0.05; ***P<0.001. Abbreviations: AUC, area under the curve; qRT-PCR, quantitative real-time PCR; ROC, receiver operating characteristic.
Figure 4
Figure 4
qRT-PCR validation in endometriosis and ovarian cancer patient cohort for miR-93, miR-141, miR-155, miR-429, miR-200c, miR-205 and miR-492. ROC curve analysis of expression levels of miRNAs for endometriosis and ovarian cancer group. The figure displays AUC for each evaluated miRNA, a parameter that indicates the precision in discriminating the endometrial tissue and ovarian cancer tissue from the normal tissue. *P<0.05; ***P<0.001. Abbreviations: AUC, area under the curve; qRT-PCR, quantitative real-time PCR; ROC, receiver operating characteristic.

Similar articles

See all similar articles

Cited by 5 articles

References

    1. Aznaurova YB, Zhumataev MB, Roberts TK, Aliper AM, Zhavoronkov AA. Molecular aspects of development and regulation of endometriosis. Reprod Biol Endocrinol. 2014;12:50. - PMC - PubMed
    1. Liu FS. Molecular carcinogenesis of endometrial cancer. Taiwan J Obstet Gynecol. 2007;46(1):26–32. - PubMed
    1. Guo SW. Endometriosis and ovarian cancer: potential benefits and harms of screening and risk-reducing surgery. Fertil Steril. 2015;104(4):813–830. - PubMed
    1. Laudanski P, Charkiewicz R, Tolwinska A, Szamatowicz J, Charkiewicz A, Niklinski J. Profiling of selected microRNAs in proliferative eutopic endometrium of women with ovarian endometriosis. Biomed Res Int. 2015;2015:760698. - PMC - PubMed
    1. Mandai M, Yamaguchi K, Matsumura N, Baba T, Konishi I. Ovarian cancer in endometriosis: molecular biology, pathology, and clinical management. Int J Clin Oncol. 2009;14(5):383–391. - PubMed

LinkOut - more resources

Feedback