Lactobacillus curtus sp. nov., isolated from beer in Finland

Int J Syst Evol Microbiol. 2017 Oct;67(10):3899-3906. doi: 10.1099/ijsem.0.002220. Epub 2017 Sep 12.


A Gram-stain-positive, catalase-negative and short-rod-shaped organism, designated VTT E-94560, was isolated from beer in Finland and deposited in the VTT culture collection as a strain of Lactobacillus rossiae. However, the results of 16S rRNA gene sequence analysis showed that VTT E-94560 was only related to Lactobacillus rossiae JCM 16176T with 97.0 % sequence similarity, lower than the 98.7 % regarded as the boundary for the species differentiation. Additional phylogenetic studies on the pheS gene, rpoA gene and 16S-23S rRNA internally transcribed spacer region further reinforced the taxonomically independent status of VTT E-94560 and its related Lactobacillus species including L. rossiae and Lactobacillus siliginis. Strain VTT E-94560 also exhibited several differences in its carbohydrate fermentation profiles from those related Lactobacillus species. In addition, DNA-DNA relatedness between VTT E-94560 and these two type strains was 4 % (L. rossiae JCM 16176T) and 12 % (L. siliginins JCM 16155T), respectively, which were lower than the 70 % cut-off for general species delineation, indicating that these three strains are not taxonomically identical at the species level. These studies revealed that VTT E-94560 represents a novel species, for which the name Lactobacillus curtus sp. nov. is proposed. The type strain is VTT E-94560T (=JCM 31185T).

Keywords: Lactobacillus cultus; beer.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Beer / microbiology*
  • DNA, Bacterial / genetics
  • Finland
  • Food Microbiology*
  • Genes, Bacterial
  • Lactobacillus / classification*
  • Lactobacillus / genetics
  • Lactobacillus / isolation & purification
  • Nucleic Acid Hybridization
  • Peptidoglycan / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA


  • DNA, Bacterial
  • Peptidoglycan
  • RNA, Ribosomal, 16S