Determination of azelastine and desmethylazelastine in human plasma by high-performance liquid chromatography

J Chromatogr. 1987 Sep 4;420(1):89-98. doi: 10.1016/0378-4347(87)80158-5.


A manual-injection liquid chromatographic method using fluorescence detection permitted determination of a new antiasthmatic drug, azelastine, and its desmethyl metabolite extracted from human plasma. Reliable quantitation was achieved to at least 0.3 ng/ml for each analyte. No interference was seen in co-chromatography of sixteen other substances, which were potential co-medications (or their metabolites) as used in standard asthma or allergy treatment.

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Drug Interactions
  • Histamine H1 Antagonists / blood*
  • Humans
  • Phthalazines / blood*
  • Pyridazines / blood*
  • Spectrometry, Fluorescence


  • Histamine H1 Antagonists
  • Phthalazines
  • Pyridazines
  • desmethylazelastine
  • azelastine