Hepatic biotransformation in lean and obese Wistar Kyoto rats: comparison to that in streptozotocin-pretreated Sprague-Dawley rats

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1987;88(1):159-64. doi: 10.1016/0742-8413(87)90061-2.


1. Phase I and phase II biotransformation was compared in streptozotocin-induced hypoinsulinemic (STZ) and genetic hyperinsulinemic (WKY-fatty) rats. 2. Total cytochrome P-450 concentrations were reduced in both STZ and WKY, whereas styrene oxide hydrolase and benzphetamine N-demethylase activities were normal in STZ and reduced in WKY. 3. UDP-glucuronosyltransferase activity was decreased toward testosterone and 1-naphthol in STZ and WKY, and was increased toward estrone in the obese female WKY. 4. Glutathione S-transferase activity was decreased in STZ toward 1-chloro-2,4-dinitrobenzene, ethacrynic acid and sulfobromophthalein, but was similar to that in normal rats for WKY.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytochrome P-450 Enzyme System / metabolism
  • Cytosol
  • Diabetes Mellitus, Experimental / enzymology*
  • Epoxide Hydrolases / metabolism
  • Glucuronosyltransferase / metabolism
  • Kinetics
  • Ligands / metabolism
  • Liver / enzymology*
  • Male
  • Microsomes, Liver / enzymology*
  • Obesity / enzymology*
  • Oxidoreductases, N-Demethylating / metabolism
  • Rats
  • Rats, Inbred WKY
  • Species Specificity


  • Ligands
  • Cytochrome P-450 Enzyme System
  • benzphetamine N-demethylase
  • Oxidoreductases, N-Demethylating
  • Glucuronosyltransferase
  • Epoxide Hydrolases
  • styrene oxide hydratase