Luteolibacter gellanilyticus sp. nov., a gellan-gum-degrading bacterium of the phylum Verrucomicrobia isolated from miniaturized diffusion chambers

Int J Syst Evol Microbiol. 2017 Oct;67(10):3951-3959. doi: 10.1099/ijsem.0.002227. Epub 2017 Sep 14.


A novel chemo-organoheterotrophic bacterium, strain CB-286403T, was isolated from a Mediterranean forest soil, collected at Sierra de Tejeda, Almijara and Alhama Natural Park, Spain, by using the Diffusion Sandwich System, a device with 384 miniature diffusion chambers. The 16S rRNA gene sequence analyses identified the isolate as a member of the genus Luteolibacter where the type strains Luteolibacterpohnpeiensis A4T-83T (GenBank acc. no. AB331895), Luteolibacteryonseiensis EBTL01T (JQ319003), Luteolibacterluojiensis DR4-30T (JN630810) and Luteolibacteralgae A5J-41-2T (AB331893) were the closest relatives with similarities of 97.0, 96.3, 96.3 and 94.5 %, respectively. The novel isolate was characterized as a Gram-stain-negative, non-motile, short-rod-shaped bacterium. The strain showed a positive response for catalase and cytochrome-c oxidase, divided by binary fission and/or budding, and exhibited an aerobic metabolism. Strain CB-286403T showed a mesophilic and neutrophilic growth range and showed a nutritional preference for simple sugars and complex protein substrates. Major fatty acids included iso-C14 : 0, C16 : 0, C16 : 1ω7c/iso-C15 : 0 2-OH and anteiso-C15 : 0. The predominant respiratory quinone was MK-9. Polar lipids comprised major amounts of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol and minor amounts of three unidentified lipids, a glycolipid, a phospholipid and a phosphoglycolipid. Based on a polyphasic taxonomic characterization, strain CB-286403T represents a novel species of the genus Luteolibacter, for which the name Luteolibacter gellanilyticus sp. nov. is proposed. The type strain is CB-286403T (=DSM 28998T=CECT 8659T).

Keywords: Luteolibacter; Verrucomicrobia; diffusion sandwich system.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Forests*
  • Phospholipids / chemistry
  • Phylogeny*
  • Polysaccharides, Bacterial / metabolism
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Spain
  • Verrucomicrobia / classification*
  • Verrucomicrobia / genetics
  • Verrucomicrobia / isolation & purification
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry


  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • Polysaccharides, Bacterial
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 9
  • gellan gum