Optimized PEI-based Transfection Method for Transient Transfection and Lentiviral Production

Curr Protoc Chem Biol. 2017 Sep 14;9(3):147-157. doi: 10.1002/cpch.25.


Polyethyleneimine (PEI), a cationic polymer vehicle, forms a complex with DNA which then can carry anionic nucleic acids into eukaryotic cells. PEI-based transfection is widely used for transient transfection of plasmid DNA. The efficiency of PEI-based transfection is affected by numerous factors, including the way the PEI/DNA complex is prepared, the ratio of PEI to DNA, the concentration of DNA, the storage conditions of PEI solutions, and more. Considering the major influencing factors, PEI-based transfection has been optimized to improve its efficiency, reproducibility, and consistency. This protocol outlines the steps for ordinary transient transfection and lentiviral production using PEI. © 2017 by John Wiley & Sons, Inc.

Keywords: lentivirial production; polyethylenimine; transfection method; transient transfection.

MeSH terms

  • DNA / genetics
  • DNA / metabolism
  • Humans
  • Lentivirus / genetics*
  • Lentivirus / growth & development*
  • Plasmids / genetics
  • Plasmids / metabolism
  • Polyethyleneimine / chemistry*
  • Transfection / methods*


  • Polyethyleneimine
  • DNA