Piracetam inhibits ethanol (EtOH)-induced memory deficit by mediating multiple pathways

Brain Res. 2017 Dec 1;1676:83-90. doi: 10.1016/j.brainres.2017.09.013. Epub 2017 Sep 11.

Abstract

Excessive ethanol (EtOH) intake, especially to prenatal exposure, can significantly affect cognitive function and cause permanent learning and memory injures in children. As a result, how to protect children from EtOH neurotoxicity has gained increasing attention in recent years. Piracetam (Pir) is a nootropic drug derived from c-aminobutyric acid and can manage cognition impairments in multiple neurological disorders. Studies have shown that Pir can exert therapeutic effects on EtOH-induced memory impairments, but the underlying mechanism is still unknown. In this study, we found that Pir inhibited ethanol-induced memory deficit by mediating multiple pathways. Treatment with EtOH could cause cognitive deficit in juvenile rats, and triggered the alteration of synaptic plasticity. Administration with Pir significantly increased long-term potentiation and protected hippocampus neurons from EtOH neurotoxicity. Pir intervention ameliorated EtOH-induced cell apoptosis and inhibited the activation of Caspase-3 in vitro, suggesting that Pir protected neurons by anti-apoptotic effects. Pir could decrease the expression of LC3-II and Beclin-1 induced by EtOH, and increase the phosphorylation of mTOR and reduce the phosphorylation of Akt, which suggested that the protective effect of Pir was involved in regulation of autophagic process and mTOR/Akt pathways. In conclusion, we speculate that Pir reduces EtOH-induced neuronal damage by regulation of apoptotic action and autophagic action, and our research offers preclinical evidence for the application of Pir in ethanol toxicity.

Keywords: Apoptosis; Autophagy; Ethanol; Hippocampus; Memory; Piracetam.

MeSH terms

  • Alcohol-Related Disorders / drug therapy*
  • Alcohol-Related Disorders / pathology
  • Alcohol-Related Disorders / physiopathology
  • Alcohol-Related Disorders / psychology
  • Animals
  • Apoptosis / drug effects
  • Apoptosis / physiology
  • Cell Line
  • Cell Survival / drug effects
  • Cell Survival / physiology
  • Dose-Response Relationship, Drug
  • Ethanol / toxicity
  • Hippocampus / drug effects
  • Hippocampus / pathology
  • Hippocampus / physiopathology
  • Long-Term Potentiation / drug effects
  • Memory Disorders / drug therapy*
  • Memory Disorders / etiology*
  • Memory Disorders / pathology
  • Memory Disorders / physiopathology
  • Mice
  • Neurons / drug effects
  • Neurons / pathology
  • Neurons / physiology
  • Neuroprotective Agents / pharmacology*
  • Nootropic Agents / pharmacology*
  • Piracetam / pharmacology*
  • Random Allocation
  • Rats, Sprague-Dawley
  • Tissue Culture Techniques

Substances

  • Neuroprotective Agents
  • Nootropic Agents
  • Ethanol
  • Piracetam