Selection of PD1/PD-L1 X-Aptamers

Biochimie. 2018 Feb;145:125-130. doi: 10.1016/j.biochi.2017.09.006. Epub 2017 Sep 11.

Abstract

Specific, chemically modified aptamers (X-Aptamers) were identified against two immune checkpoint proteins, recombinant Programmed Death 1 (PD-1) and Programmed Death Ligand 1 (PD-L1). Selections were performed using a bead-based X-Aptamer (XA) library containing several different amino acid functional groups attached to dU at the 5-position. The binding affinities and specificities of the selected XA-PD1 and XA-PDL1 were validated by hPD-1 and hPD-L1 expression cells, as well as by binding to human pancreatic ductal adenocarcinoma tissue. The selected PD1 and PDL1 XAs can mimic antibody functions in in vitro assays.

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / pathology
  • Animals
  • Aptamers, Nucleotide* / chemistry
  • Aptamers, Nucleotide* / pharmacokinetics
  • Aptamers, Nucleotide* / pharmacology
  • B7-H1 Antigen / antagonists & inhibitors*
  • Cell Line
  • Humans
  • Mice
  • Neoplasm Proteins / antagonists & inhibitors*
  • Neoplasm Proteins / metabolism
  • Pancreatic Neoplasms / metabolism*
  • Pancreatic Neoplasms / pathology
  • Programmed Cell Death 1 Receptor / antagonists & inhibitors*

Substances

  • Aptamers, Nucleotide
  • B7-H1 Antigen
  • CD274 protein, human
  • Neoplasm Proteins
  • PDCD1 protein, human
  • Programmed Cell Death 1 Receptor