Complete Genome Sequence of Dehalobacterium formicoaceticum Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium

Gao Chen; Robert W Murdoch; E Erin Mack; Edward S Seger; Frank E Löffler

doi:10.1128/genomeA.00897-17

Complete Genome Sequence of Dehalobacterium formicoaceticum Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium

Genome Announc. 2017 Sep 14;5(37):e00897-17. doi: 10.1128/genomeA.00897-17.

Authors

Gao Chen^{1

2}, Robert W Murdoch¹, E Erin Mack³, Edward S Seger⁴, Frank E Löffler^{5

2

6

7

8}

Affiliations

¹ Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA.
² Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee, USA.
³ Corporate Remediation Group, E. I. DuPont de Nemours and Company, Newark, Delaware, USA.
⁴ The Chemours Company, Wilmington, Delaware, USA.
⁵ Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA frank.loeffler@utk.edu.
⁶ Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA.
⁷ Department of Biosystems Engineering and Soil Science, University of Tennessee, Knoxville, Tennessee, USA.
⁸ Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.

Abstract

Dehalobacterium formicoaceticum utilizes dichloromethane as the sole energy source in defined anoxic bicarbonate-buffered mineral salt medium. The products are formate, acetate, inorganic chloride, and biomass. The bacterium's genome was sequenced using PacBio, assembled, and annotated. The complete genome consists of one 3.77-Mb circular chromosome harboring 3,935 predicted protein-encoding genes.