Developmental Cycle and Genome Analysis of Protochlamydia massiliensis sp. nov. a New Species in the Parachlamydiacae Family

Front Cell Infect Microbiol. 2017 Aug 31;7:385. doi: 10.3389/fcimb.2017.00385. eCollection 2017.


Amoeba-associated microorganisms (AAMs) are frequently isolated from water networks. In this paper, we report the isolation and characterization of Protochlamydia massiliensis, an obligate intracellular Gram-negative bacterium belonging to the Parachlamydiaceae family in the Chlamydiales order, from a cooling water tower. This bacterium was isolated on Vermamoeba vermiformis. It has a multiple range of hosts among amoeba and is characterized by a typical replication cycle of Chlamydiae with a particularity, recently shown in some chlamydia, which is the absence of inclusion vacuoles in the V. vermiformis host, adding by this a new member of Chlamydiae undergoing developmental cycle changes in the newly adapted host V. vermiformis. Draft genome sequencing revealed a chromosome of 2.86 Mb consisting of four contigs and a plasmid of 92 Kb.

Keywords: CRISPR; Chlamydiae; Protochlamydia massiliensis; Vermamoeba vermiformis; co-culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amoeba / microbiology
  • Chlamydiales / classification
  • Chlamydiales / genetics*
  • Chlamydiales / growth & development*
  • Chlamydiales / isolation & purification
  • Coculture Techniques
  • DNA, Bacterial / genetics
  • Genome / genetics*
  • Phylogeny
  • Plasmids
  • RNA, Ribosomal / genetics
  • Vacuoles / microbiology
  • Whole Genome Sequencing


  • DNA, Bacterial
  • RNA, Ribosomal