Hox-1.6: a mouse homeo-box-containing gene member of the Hox-1 complex

EMBO J. 1987 Oct;6(10):2977-86.

Abstract

Hox-1.6, a mouse homeo-box-containing gene member of the Hox-1 complex, is described. The Hox-1.6 homeo-box shows more divergence than the other members of the complex with the Drosophila Antennapedia-like homeo-box class. This previously undescribed gene was studied with respect to its transcription pattern and was found to be expressed during mouse fetal development in an intestine-specific manner in adults, and in tumours or cell types exhibiting early endodermal-like differentiation. The study of embryonic partial Hox-1.6 cDNA clones revealed structural features common to other Drosophila and vertebrate homeo-box-containing genes, but also indicated that Hox-1.6 transcripts might display splicing patterns more complex than those known for other vertebrate homeo-genes. One of these cDNA clones contains a rather short open reading frame which would encode a protein of approximately 14.5 kd. The use of this clone as a probe for S1 nuclease mapping confirmed that different Hox-1.6 transcripts were present both in embryonic total RNA and in embryonal carcinoma cell cytoplasmic RNA. These various transcripts are probably generated by an alternative splicing mechanism and may thus encode a set of related proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cosmids
  • DNA / analysis
  • Drosophila / genetics
  • Embryo, Mammalian
  • Embryo, Nonmammalian
  • Genes, Homeobox*
  • Genetic Variation*
  • Mice / genetics*
  • Molecular Sequence Data
  • RNA Splicing
  • Transcription, Genetic

Substances

  • DNA

Associated data

  • GENBANK/M15926
  • GENBANK/M15927
  • GENBANK/M15928
  • GENBANK/M20214
  • GENBANK/M20215
  • GENBANK/M20216
  • GENBANK/X06023
  • GENBANK/X06024