Nano-hemoglobin film based sextet state biomemory device by cross-linked photosensitive hapten monomer

Talanta. 2018 Jan 1:176:85-91. doi: 10.1016/j.talanta.2017.08.014. Epub 2017 Aug 5.

Abstract

In this study, a biomemory device, consisting of hemoglobin (Hb) cross-linked by MACys-Ru(bipyr)2-MACys) photosensitive monomer cross-linkers, which have memory effect through both Ru3+/2+ in hapten monomer and Fe3+/2+ in redox active center of Hb through multi-charge transfer mechanism, has been improved. Cyclic voltammetry (CV) has been used to determine the redox property of the Hb cross-linked MACys-Ru(bipyr)2-MACys) hapten. Three memory functions, writing, reading and erasing of the fabricated biomemory device, have been accomplished by chronoamperometry (CA) and open-circuit potential amperometry (OCPA). The reliability and repeatability of the biodevice consisting of the p(Hb-co-MACys-Ru(bipyr)2-MACys) sextet state bio-memory layer have been analysed. The Hb film based biodevice on gold electrodes has shown ≥ 2 months the retention time and switched until 106 times continuous cycling without degradation in efficiency. Other hand, the topography of p(Hb-co-MACys-Ru(bipyr)2-MACys) layer on the gold surface has investigated by scanning electron microscopy (SEM) and EDX data.

Keywords: Biomemory device; Chronoamperometry; Hemoglobine; Nanobiochip; Photosensitive monomer.

MeSH terms

  • Ammonium Sulfate / chemistry
  • Cysteine / analogs & derivatives
  • Cysteine / chemistry
  • Gold / chemistry
  • Haptens / chemistry
  • Hemoglobins / chemistry*
  • Nanostructures / chemistry*
  • Organometallic Compounds / chemistry

Substances

  • Haptens
  • Hemoglobins
  • Organometallic Compounds
  • bis(ruthenium-bipyridine)
  • ammonium peroxydisulfate
  • Gold
  • Cysteine
  • Ammonium Sulfate