Skin regeneration effect of the Glycosaminoglycans from Liparis tessellatus eggs

Int J Biol Macromol. 2017 Dec;105(Pt 2):1369-1374. doi: 10.1016/j.ijbiomac.2017.08.182. Epub 2017 Sep 14.


A purified Glycosaminoglycans (GAGs) extract from L. tessellatus eggs was enzyme-hydrolyzed and then fractionated with DEAE-Sepharose column chromatography. The fractions were subsequently subjected to skin regeneration effects analysis against skin fibroblast (CCD-986sk) cell lines. Fraction 3 is evidently to have tyrosinase inhibition activity by 20.1% at concentration of 50mg/mL. DOPA oxidation assay, collagenase inhibition activity assay, fibroblast proliferation assay, and production of type I C-peptide assays were done to further proof the skin regeneration effect of GAGs fractions. Results revealed that fraction 3 has effective skin regeneration activities at a concentration of 200mg/mL.

Keywords: Fibroblast proliferation; Glycosaminoglycans; Liparis tessellatus eggs; Tyrosinase inhibition.

MeSH terms

  • Animals
  • Cell Line
  • Cell Proliferation / drug effects
  • Collagen Type I / biosynthesis
  • Collagenases / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fishes*
  • Glycosaminoglycans / pharmacology*
  • Monophenol Monooxygenase / antagonists & inhibitors
  • Ovum / chemistry*
  • Regeneration / drug effects*
  • Skin / cytology
  • Skin / drug effects*


  • Collagen Type I
  • Glycosaminoglycans
  • Monophenol Monooxygenase
  • Collagenases