Exosomes derived from human umbilical cord blood mesenchymal stem cells stimulates rejuvenation of human skin

Biochem Biophys Res Commun. 2017 Nov 18;493(2):1102-1108. doi: 10.1016/j.bbrc.2017.09.056. Epub 2017 Sep 14.


Human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) play an important role in cutaneous wound healing, and recent studies suggested that MSC-derived exosomes activate several signaling pathways, which are conducive in wound healing and cell growth. In this study, we investigated the roles of exosomes that are derived from USC-CM (USC-CM Exos) in cutaneous collagen synthesis and permeation. We found that USC-CM has various growth factors associated with skin rejuvenation. Our in vitro results showed that USC-CM Exos integrate in Human Dermal Fibroblasts (HDFs) and consequently promote cell migration and collagen synthesis of HDFs. Moreover, we evaluated skin permeation of USC-CM Exos by using human skin tissues. Results showed that Exo-Green labeled USC-CM Exos approached the outermost layer of the epidermis after 3 h and gradually approached the epidermis after 18 h. Moreover, increased expressions of Collagen I and Elastin were found after 3 days of treatment on human skin. The results showed that USC-CM Exos is absorbed into human skin, it promotes Collagen I and Elastin synthesis in the skin, which are essential to skin rejuvenation and shows the potential of USC-CM integration with the cosmetics or therapeutics.

Keywords: Conditioned media from hUCB-MSCs; Cosmetics; Exosomes; Skin permeation; USC-CM.

MeSH terms

  • Adult
  • Cells, Cultured
  • Collagen / metabolism
  • Cosmetics
  • Elastin / metabolism
  • Exosomes / chemistry
  • Exosomes / metabolism*
  • Female
  • Fetal Blood / chemistry
  • Fetal Blood / cytology*
  • Fetal Blood / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • Intercellular Signaling Peptides and Proteins / isolation & purification
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Intercellular Signaling Peptides and Proteins / pharmacokinetics
  • Intercellular Signaling Peptides and Proteins / pharmacology*
  • Mesenchymal Stem Cells / chemistry
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Rejuvenation*
  • Skin Absorption
  • Skin Physiological Phenomena* / drug effects


  • Cosmetics
  • Intercellular Signaling Peptides and Proteins
  • Collagen
  • Elastin