Leukocyte-reduced platelet-rich plasma stimulates the in vitro proliferation of adipose-tissue derived mesenchymal stem cells depending on PDGF signaling

Clin Hemorheol Microcirc. 2017;67(2):183-196. doi: 10.3233/CH-170246.


Background: Platelet-rich Plasma (PRP) is suggested as xenoprotein-free cell-culture medium replacement for animal-derived supplements.

Objective: The aim of this study was to investigate PRP-triggered signaling in adipose derived mesenchymal stem cells (ASCs).

Methods: PRP was obtained from 4 male patients. We incubated ASCs in α-MEM with different Platelet derived growth factor (PDGF) subtypes or 10% or 20% pooled PRP or 20% fetal calf serum (FCS) prior to determination of the S-phase fraction (SPF). To investigate the influence of PDGF signaling on ASCs, PDGF receptor β inhibitor was added, and protein expression of ASCs was measured.

Results: ASCs exposed to 20% PRP, PDGF-AB and - BB demonstrated significant higher SPF in comparison to PDGF-AA and 20% FCS after 48 hours (all P < 0.05). PDGF receptor β inhibition diminished the PRP-induced SPF increase of ASCs significantly after 48 hours (P < 0.01). ASCs with PDGF receptor β inhibition showed significant higher PDGF receptor β and significant lower c-MYC expression compared to untreated cells in presence of 20% PRP after 48 hours (both P < 0.05).

Conclusions: The proliferation promoting effect of PRP on ASCs is mediated by PDGF signaling and is associated with c-MYC overexpression.

Keywords: PDGF receptor β inhibition; Platelet-rich Plasma; cell culture; mesenchymal stem cells; proliferation.

MeSH terms

  • Cell Culture Techniques
  • Cell Proliferation
  • Female
  • Humans
  • Leukocytes / metabolism*
  • Male
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Platelet-Derived Growth Factor / metabolism*
  • Platelet-Rich Plasma / metabolism*


  • Platelet-Derived Growth Factor
  • platelet-derived growth factor A